Eurofins TRAC® ID

Eurofins TRAC® ID is the Next Evolution in Molecular Biomarkers

Donor Derived Cell Free DNA is something most nephrologists are comfortable using in post-transplant testing. It is a marker of injury, that tells us that the transplanted kidney is in distress. But it does not tell us why. 

Introducing TRAC ID – the first ddCFDNA test that utilizes whole genome sequencing to analyze not only the human sequences to calculate the donor derived fraction of cfDNA, but also analyzes the microbial sequences of viral infections. The addition of the ID panel provides additional information to guide decisions on immunosuppression titration and future patient monitoring strategy.

What is ID?

The current industry standard method for allograft rejection diagnosis and surveillance is organ biopsy, an invasive technique that suffers from high cost and carries the risk of serious complications for the patient. Biopsies may also be inconclusive and lead to adverse treatment decisions. Quantification of dd-cfDNA by NGS methods in a transplant recipient’s plasma has been developed to non-invasively monitor and aid in the diagnosis of organ rejection in transplant recipients. Donor-derived cell-free DNA is released normally through natural processes like apoptosis but is observed to increase in concentration in response to an immune response (either antibody or t-cell mediated) against the donated organ.

Eurofins TRAC ID utilizes whole genome sequencing (WGS) to assess dd-cfDNA. This approach enables the quantification of non-human (viral) DNA in addition to the assessment of donor vs. recipient DNA.

Combined Metagenomic Viral Detection and Donor-Derived Cell-Free DNA Quantification in Plasma From Kidney Transplant Recipients

Rohita Sinhaa, Zixuan Zhua, Sookhyeon Parkb, Christabel Rebellob, Bradley Kinsellab,
John Friedewaldb, and Steven Kleiboekera*


a Eurofins Viracor Clinical Diagnostics, Lenexa, Kansas; and b Northwestern University, Feinberg School of Medicine, Chicago, Illinois

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